SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07188

Signal sequence   MKQGKFSVFLILLLMLTLVVAPKGKAEA

Length  28

Organism  Bacillus subtilis 1A751

UniProt ID  NA,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 18(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07188.cif

Structure  SPKB07188.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3046.85004

Aliphatic index  139.2857143

Isoelectric point  10.80270634

Charge (pH=7)   2.998579287

Topological polar surface area  1065.16

Aromaticity  0.071428571

Average rotatable bonds  3.75

Number of hydrogen bond donors  36

Number of hydrogen bond acceptors  39

X logP energy  -3.3817

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.