General Information
SPKB ID SPKB07187
Signal sequence MKKKKTWKRFLHFSSAALAAGLIFTSAAPAEA
Length 32
Organism Bacillus subtilis 1A751
UniProt ID P94522,
Motif 1..32
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 18.1(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07187.cif
Physicochemical Information
Molecular weight 3479.14684
Aliphatic index 73.75
Isoelectric point 11.36666267
Charge (pH=7) 5.089190032
Topological polar surface area 1331.55
Aromaticity 0.125
Average rotatable bonds 3.59375
Number of hydrogen bond donors 48
Number of hydrogen bond acceptors 47
X logP energy -10.40913
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
