General Information
SPKB ID SPKB07186
Signal sequence MKTLRTLCVLMILSGVIFFGLKIDA
Length 25
Organism Bacillus subtilis 1A751
UniProt ID O34735,
Motif 1..25
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 29.7(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07186.cif
Physicochemical Information
Molecular weight 2783.52814
Aliphatic index 152
Isoelectric point 9.673752869
Charge (pH=7) 1.935862689
Topological polar surface area 973.65
Aromaticity 0.08
Average rotatable bonds 3.8
Number of hydrogen bond donors 36
Number of hydrogen bond acceptors 36
X logP energy -3.33173
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
