General Information
SPKB ID SPKB07184
Signal sequence MNKFLKSNFRFLLAAALGISLLASSNFIKA
Length 30
Organism Bacillus subtilis 1A751
UniProt ID NA,
Motif 1..30
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 96.1(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07184.cif
Physicochemical Information
Molecular weight 3286.96934
Aliphatic index 120.6666667
Isoelectric point 11.92450937
Charge (pH=7) 3.997095887
Topological polar surface area 1257.37
Aromaticity 0.133333333
Average rotatable bonds 3.733333333
Number of hydrogen bond donors 44
Number of hydrogen bond acceptors 43
X logP energy -9.47323
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
