General Information
SPKB ID SPKB07183
Signal sequence MKKIVSILFMFGLVMGFSQFQPSTVFA
Length 27
Organism Bacillus subtilis 1A751
UniProt ID O34310,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 177.9(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07183.cif
Physicochemical Information
Molecular weight 3054.76004
Aliphatic index 93.7037037
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 1030.27
Aromaticity 0.185185185
Average rotatable bonds 3.703703704
Number of hydrogen bond donors 35
Number of hydrogen bond acceptors 39
X logP energy -4.4361
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
