SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07182

Signal sequence   MLKKVILAAFILVGSTLGAFSFSSDASA

Length  28

Organism  Bacillus subtilis 1A751

UniProt ID  NA,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 179.4(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07182.cif

Structure  SPKB07182.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2845.38994

Aliphatic index  122.1428571

Isoelectric point  9.537297863

Charge (pH=7)   0.997840716

Topological polar surface area  1059.74

Aromaticity  0.107142857

Average rotatable bonds  3.357142857

Number of hydrogen bond donors  38

Number of hydrogen bond acceptors  39

X logP energy  -9.005

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.