SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07181

Signal sequence   MHLIRAAGAVCLAVVLIAGCRFNEDQHQAEG

Length  31

Organism  Bacillus subtilis 1A751

UniProt ID  NA,

Motif  1..31



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 234(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07181.cif

Structure  SPKB07181.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3293.82734

Aliphatic index  110.3225806

Isoelectric point  6.487111004

Charge (pH=7)   -0.940156844

Topological polar surface area  1358.65

Aromaticity  0.032258065

Average rotatable bonds  3.516129032

Number of hydrogen bond donors  48

Number of hydrogen bond acceptors  45

X logP energy  -12.92136

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.