General Information
SPKB ID SPKB07176
Signal sequence MKKLLAAGIIGLLTVSIASPSFA
Length 23
Organism Bacillus subtilis 1A751
UniProt ID P70961,
Motif 1..23
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 447.5(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07176.cif
Physicochemical Information
Molecular weight 2301.85644
Aliphatic index 148.6956522
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 827.69
Aromaticity 0.043478261
Average rotatable bonds 3.304347826
Number of hydrogen bond donors 29
Number of hydrogen bond acceptors 31
X logP energy -5.4973
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
