SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07173

Signal sequence   MKKRITYSLLALLAVVAFAFTDSSKAKA

Length  28

Organism  Bacillus subtilis 1A751

UniProt ID  O31994,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 473.4(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07173.cif

Structure  SPKB07173.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3044.68934

Aliphatic index  111.7857143

Isoelectric point  10.85742466

Charge (pH=7)   3.996396923

Topological polar surface area  1173.68

Aromaticity  0.107142857

Average rotatable bonds  3.678571429

Number of hydrogen bond donors  43

Number of hydrogen bond acceptors  42

X logP energy  -8.67093

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.