SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07171

Signal sequence   MRIQKRRTHVENILRILLPPIMILSLILPTPPIHA

Length  35

Organism  Bacillus subtilis 1A751

UniProt ID  O34313,

Motif  1..35



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  alpha-amylase yield: 512.4(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07171.cif

Structure  SPKB07171.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4095.11224

Aliphatic index  156

Isoelectric point  12.50839109

Charge (pH=7)   4.181269325

Topological polar surface area  1523.92

Aromaticity  0

Average rotatable bonds  3.771428571

Number of hydrogen bond donors  52

Number of hydrogen bond acceptors  51

X logP energy  -6.93282

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.