General Information
SPKB ID SPKB07170
Signal sequence MRIFKKAVFVIMISFLIATVNVNTAHA
Length 27
Organism Bacillus subtilis 1A751
UniProt ID P35150,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 548.9(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07170.cif
Physicochemical Information
Molecular weight 3035.74564
Aliphatic index 130
Isoelectric point 11.82306209
Charge (pH=7) 3.088300011
Topological polar surface area 1109.41
Aromaticity 0.111111111
Average rotatable bonds 3.703703704
Number of hydrogen bond donors 39
Number of hydrogen bond acceptors 39
X logP energy -5.83673
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
