General Information
SPKB ID SPKB07169
Signal sequence MKNMSCKLVVSVTLFFSFLTIGPLAHA
Length 27
Organism Bacillus subtilis 1A751
UniProt ID P16396,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 565.7(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07169.cif
Physicochemical Information
Molecular weight 2955.62794
Aliphatic index 111.8518519
Isoelectric point 9.677346754
Charge (pH=7) 2.026328607
Topological polar surface area 1036.09
Aromaticity 0.111111111
Average rotatable bonds 3.518518519
Number of hydrogen bond donors 37
Number of hydrogen bond acceptors 40
X logP energy -6.6192
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
