SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07166

Signal sequence   MNTLANWKKFLLVAVIICFLVPIMTKAEIAEA

Length  32

Organism  Bacillus subtilis 1A751

UniProt ID  P96740,

Motif  1..32



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 632(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07166.cif

Structure  SPKB07166.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3592.46044

Aliphatic index  140.3125

Isoelectric point  8.535917

Charge (pH=7)   0.938674727

Topological polar surface area  1251.72

Aromaticity  0.09375

Average rotatable bonds  3.6875

Number of hydrogen bond donors  43

Number of hydrogen bond acceptors  45

X logP energy  -3.356

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.