General Information
SPKB ID SPKB07164
Signal sequence MKRRKFSSVVAAVLIFALIFSLFSPGTKAAA
Length 31
Organism Bacillus subtilis 1A751
UniProt ID P54423,
Motif 1..31
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency alpha-amylase yield: 668.5(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07164.cif
Physicochemical Information
Molecular weight 3328.06564
Aliphatic index 110.3225806
Isoelectric point 12.54607567
Charge (pH=7) 4.997092575
Topological polar surface area 1230.54
Aromaticity 0.129032258
Average rotatable bonds 3.516129032
Number of hydrogen bond donors 45
Number of hydrogen bond acceptors 43
X logP energy -8.61476
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
