SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07163

Signal sequence   MKRSISIFITCLLITLLTMGGMIASPASA

Length  29

Organism  Bacillus subtilis 1A751

UniProt ID  P10475,

Motif  1..29



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 706.3(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07163.cif

Structure  SPKB07163.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3040.78964

Aliphatic index  131.3793103

Isoelectric point  9.833242293

Charge (pH=7)   1.935711238

Topological polar surface area  1098.86

Aromaticity  0.034482759

Average rotatable bonds  3.482758621

Number of hydrogen bond donors  41

Number of hydrogen bond acceptors  43

X logP energy  -8.84803

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.