General Information
SPKB ID SPKB07160
Signal sequence MLKKKWMVGLLAGCLAAGGFSYNAFA
Length 26
Organism Bacillus subtilis 1A751
UniProt ID O35012,
Motif 1..26
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 789.3(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07160.cif
Physicochemical Information
Molecular weight 2748.35774
Aliphatic index 90.38461538
Isoelectric point 10.13347695
Charge (pH=7) 2.934274068
Topological polar surface area 968.22
Aromaticity 0.153846154
Average rotatable bonds 3.461538462
Number of hydrogen bond donors 35
Number of hydrogen bond acceptors 36
X logP energy -5.1678
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
