SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07156

Signal sequence   MKLKTKASIKFGICVGLLCLSITGFTPFFNSTHAEA

Length  36

Organism  Bacillus subtilis 1A751

UniProt ID  P39824,

Motif  1..36



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 889.6(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07156.cif

Structure  SPKB07156.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3875.66084

Aliphatic index  92.22222222

Isoelectric point  9.601448848

Charge (pH=7)   2.965538946

Topological polar surface area  1427.79

Aromaticity  0.111111111

Average rotatable bonds  3.555555556

Number of hydrogen bond donors  52

Number of hydrogen bond acceptors  54

X logP energy  -11.8893

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.