General Information
SPKB ID SPKB07153
Signal sequence MNIKKFAKQATVLTFTTALLAGGATQAFA
Length 29
Organism Bacillus subtilis 1A751
UniProt ID P05655,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 935.3(U/mL)
Type Sec/SPI
Structure Information
PDB ID 3BYJ,
Predicted Structure ID SPKB07153.cif
Physicochemical Information
Molecular weight 3014.57624
Aliphatic index 87.93103448
Isoelectric point 11.10307074
Charge (pH=7) 2.997099198
Topological polar surface area 1186.6
Aromaticity 0.103448276
Average rotatable bonds 3.448275862
Number of hydrogen bond donors 41
Number of hydrogen bond acceptors 42
X logP energy -11.6062
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
