SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07152

Signal sequence   MTKKAWFLPLVCVLLISGWLAPAASASA

Length  28

Organism  Bacillus subtilis 1A751

UniProt ID  NA,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 944.2(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07152.cif

Structure  SPKB07152.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2945.61724

Aliphatic index  125.7142857

Isoelectric point  9.676237541

Charge (pH=7)   1.935419516

Topological polar surface area  995.98

Aromaticity  0.107142857

Average rotatable bonds  3.178571429

Number of hydrogen bond donors  36

Number of hydrogen bond acceptors  37

X logP energy  -4.171

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.