General Information
SPKB ID SPKB07151
Signal sequence MKIRKILLSSALSFGMLISAVPALA
Length 25
Organism Bacillus subtilis 1A751
UniProt ID O31836,
Motif 1..25
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 1110.8(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07151.cif
Physicochemical Information
Molecular weight 2631.31714
Aliphatic index 152.4
Isoelectric point 11.82305527
Charge (pH=7) 2.99739092
Topological polar surface area 947.79
Aromaticity 0.04
Average rotatable bonds 3.56
Number of hydrogen bond donors 34
Number of hydrogen bond acceptors 35
X logP energy -5.48513
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
