General Information
SPKB ID SPKB07149
Signal sequence MKRMIVRMTLPLLIVCLAFSSFSASARA
Length 28
Organism Bacillus subtilis 1A751
UniProt ID O31455,
Motif 1..28
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 1175.8(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07149.cif
Physicochemical Information
Molecular weight 3113.89304
Aliphatic index 118.5714286
Isoelectric point 12.22275045
Charge (pH=7) 3.935704616
Topological polar surface area 1153.1
Aromaticity 0.071428571
Average rotatable bonds 3.678571429
Number of hydrogen bond donors 44
Number of hydrogen bond acceptors 42
X logP energy -8.39439
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
