General Information
SPKB ID SPKB07147
Signal sequence MKKKQVMLALTAAAGLGLTALHSAPAAKA
Length 29
Organism Bacillus subtilis 1A751
UniProt ID O31579,
Motif 1..29
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 1278.8(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07147.cif
Physicochemical Information
Molecular weight 2863.51634
Aliphatic index 108.2758621
Isoelectric point 11.27834369
Charge (pH=7) 4.087713255
Topological polar surface area 1105.87
Aromaticity 0
Average rotatable bonds 3.344827586
Number of hydrogen bond donors 38
Number of hydrogen bond acceptors 41
X logP energy -10.1853
Hydropathy 
Literature Information
Title Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis
DOI NA,
Publication Year 2017
PubMed ID NA,
Patent ID NA,
