SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07146

Signal sequence   MPYLKRVLLLLVTGLFMSLFAVTATASA

Length  28

Organism  Bacillus subtilis 1A751

UniProt ID  P04957,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 1350.3(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07146.cif

Structure  SPKB07146.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3027.76034

Aliphatic index  142.8571429

Isoelectric point  10.45492462

Charge (pH=7)   1.99683554

Topological polar surface area  1049.53

Aromaticity  0.107142857

Average rotatable bonds  3.428571429

Number of hydrogen bond donors  38

Number of hydrogen bond acceptors  39

X logP energy  -4.54503

Hydropathy 



Literature Information


Title  Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis

DOI  NA,

Publication Year  2017

PubMed ID  NA,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.