General Information
SPKB ID SPKB07133
Signal sequence MKKKIVAGLAVSAVVGSSMAAAPAEA
Length 26
Organism Bacillus subtilis RIK1285
UniProt ID O31852,
Motif 1..26
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 212.4(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07133.cif
Physicochemical Information
Molecular weight 2457.97294
Aliphatic index 105.3846154
Isoelectric point 10.506919
Charge (pH=7) 1.998874321
Topological polar surface area 958.08
Aromaticity 0
Average rotatable bonds 3.153846154
Number of hydrogen bond donors 33
Number of hydrogen bond acceptors 36
X logP energy -10.1024
Hydropathy 
Literature Information
Title Enhanced extracellular expression of Bacillus stearothermophilus alpha-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and alpha-amylase mutant selection
DOI 10.1186/s12934-019-1119-8,
Publication Year 2019
PubMed ID 30971250,
Patent ID NA,
