General Information
SPKB ID SPKB07132
Signal sequence MKLAKRVSALTPSTTLAITAKA
Length 22
Organism Bacillus subtilis RIK1285
UniProt ID NA,
Motif 1..22
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 246.1(U/ml)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07132.cif
Physicochemical Information
Molecular weight 2272.77534
Aliphatic index 106.8181818
Isoelectric point 11.92450937
Charge (pH=7) 3.997095887
Topological polar surface area 926.97
Aromaticity 0
Average rotatable bonds 3.454545455
Number of hydrogen bond donors 34
Number of hydrogen bond acceptors 34
X logP energy -10.06363
Hydropathy 
Literature Information
Title Enhanced extracellular expression of Bacillus stearothermophilus alpha-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and alpha-amylase mutant selection
DOI 10.1186/s12934-019-1119-8,
Publication Year 2019
PubMed ID 30971250,
Patent ID NA,
