General Information
SPKB ID SPKB07131
Signal sequence MRKKITLACKTCGNRNYTTMKSSASA
Length 26
Organism Bacillus subtilis RIK1285
UniProt ID NA,
Motif 1..26
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency alpha-amylase yield: 329.9(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07131.cif
Physicochemical Information
Molecular weight 2865.39514
Aliphatic index 41.53846154
Isoelectric point 10.85245089
Charge (pH=7) 5.871997695
Topological polar surface area 1266.72
Aromaticity 0.038461538
Average rotatable bonds 3.846153846
Number of hydrogen bond donors 49
Number of hydrogen bond acceptors 47
X logP energy -18.09086
Hydropathy 
Literature Information
Title Enhanced extracellular expression of Bacillus stearothermophilus alpha-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and alpha-amylase mutant selection
DOI 10.1186/s12934-019-1119-8,
Publication Year 2019
PubMed ID 30971250,
Patent ID NA,
