SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07104

Signal sequence   MKKMLMLAFTFLLALTIHVGEASA

Length  24

Organism  Bacillus subtilis 1A751p

UniProt ID  O34541,

Motif  1..24



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 871(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07104.cif

Structure  SPKB07104.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2637.29554

Aliphatic index  126.25

Isoelectric point  9.539374794

Charge (pH=7)   1.090078445

Topological polar surface area  911.33

Aromaticity  0.083333333

Average rotatable bonds  3.708333333

Number of hydrogen bond donors  32

Number of hydrogen bond acceptors  35

X logP energy  -3.2205

Hydropathy 



Literature Information


Title  Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis

DOI  10.1021/acs.jafc.8b04183,

Publication Year  2018

PubMed ID  30463403,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.