SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07103

Signal sequence   MRKKRVITCVMAASLTLGSLLPAGYASA

Length  28

Organism  Bacillus subtilis 1A751p

UniProt ID  P96657,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 882.05(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07103.cif

Structure  SPKB07103.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2909.55994

Aliphatic index  108.2142857

Isoelectric point  10.88840969

Charge (pH=7)   3.934562479

Topological polar surface area  1137.45

Aromaticity  0.035714286

Average rotatable bonds  3.428571429

Number of hydrogen bond donors  43

Number of hydrogen bond acceptors  42

X logP energy  -11.19376

Hydropathy 



Literature Information


Title  Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis

DOI  10.1021/acs.jafc.8b04183,

Publication Year  2018

PubMed ID  30463403,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.