SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07094

Signal sequence   MKKVLMAFIICLSLILSVLAAPPSGAKA

Length  28

Organism  Bacillus subtilis 1A751p

UniProt ID  Q79F14,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 1238.25(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07094.cif

Structure  SPKB07094.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2886.69244

Aliphatic index  150

Isoelectric point  10.51301932

Charge (pH=7)   2.935124482

Topological polar surface area  970.19

Aromaticity  0.035714286

Average rotatable bonds  3.392857143

Number of hydrogen bond donors  34

Number of hydrogen bond acceptors  38

X logP energy  -4.0454

Hydropathy 



Literature Information


Title  Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis

DOI  10.1021/acs.jafc.8b04183,

Publication Year  2018

PubMed ID  30463403,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.