SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07089

Signal sequence   MKKVMLATALFLGLTPAGANA

Length  21

Organism  Bacillus subtilis 1A751p

UniProt ID  P39116,

Motif  1..21



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  alpha-amylase yield: 1487.85(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  1BN8,

Predicted Structure ID  SPKB07089.cif

Structure  SPKB07089.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2118.62394

Aliphatic index  111.9047619

Isoelectric point  10.80537956

Charge (pH=7)   1.997394232

Topological polar surface area  772.12

Aromaticity  0.047619048

Average rotatable bonds  3.285714286

Number of hydrogen bond donors  26

Number of hydrogen bond acceptors  29

X logP energy  -5.5529

Hydropathy 



Literature Information


Title  Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis

DOI  10.1021/acs.jafc.8b04183,

Publication Year  2018

PubMed ID  30463403,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.