SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07074

Signal sequence   MKLFNRKVTLVSLILMAVFQFFMALIIKRIVIS

Length  33

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..33



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline protease yield: 7988(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07074.cif

Structure  SPKB07074.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3884.97474

Aliphatic index  159.3939394

Isoelectric point  12.54607567

Charge (pH=7)   4.997092575

Topological polar surface area  1343.25

Aromaticity  0.121212121

Average rotatable bonds  4.060606061

Number of hydrogen bond donors  48

Number of hydrogen bond acceptors  47

X logP energy  -2.31336

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.