SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07072

Signal sequence   MKRHYEAVVIGGGIIGSAIAYYLAKENKNTALFESGTMGGRTTSAAAG

Length  48

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..48



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline protease yield: 8632.3(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  1NG3,

Predicted Structure ID  SPKB07072.cif

Structure  SPKB07072.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4934.61974

Aliphatic index  77.5

Isoelectric point  9.698068763

Charge (pH=7)   2.090775792

Topological polar surface area  2061.94

Aromaticity  0.083333333

Average rotatable bonds  3.4375

Number of hydrogen bond donors  74

Number of hydrogen bond acceptors  72

X logP energy  -23.69866

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.