SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07069

Signal sequence   MLFNQRRGISPAALIIGSTMLITALSPQIRQRI

Length  33

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..33



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline protease yield: 8848.8(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07069.cif

Structure  SPKB07069.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3667.43534

Aliphatic index  127.2727273

Isoelectric point  12.97714556

Charge (pH=7)   3.997971054

Topological polar surface area  1498.05

Aromaticity  0.03030303

Average rotatable bonds  3.727272727

Number of hydrogen bond donors  53

Number of hydrogen bond acceptors  49

X logP energy  -14.07982

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.