General Information
SPKB ID SPKB07065
Signal sequence MKFVKRRIIALVTILMLSVTSLFALQPSAKA
Length 31
Organism Bacillus amyloliquefaciens 111018
UniProt ID NA,
Motif 1..31
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline protease yield: 10060.56(U/mL)
Type Tat/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07065.cif
Physicochemical Information
Molecular weight 3447.33134
Aliphatic index 141.6129032
Isoelectric point 12.54607567
Charge (pH=7) 4.997092575
Topological polar surface area 1273.63
Aromaticity 0.064516129
Average rotatable bonds 3.774193548
Number of hydrogen bond donors 46
Number of hydrogen bond acceptors 45
X logP energy -7.22596
Hydropathy
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,