SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07062

Signal sequence   MPYLKRVLLLLVTGLFMSLFAVTATASAQ

Length  29

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..29



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline protease yield: 10468.28(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07062.cif

Structure  SPKB07062.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3155.89104

Aliphatic index  137.9310345

Isoelectric point  10.45492462

Charge (pH=7)   1.99683554

Topological polar surface area  1121.72

Aromaticity  0.103448276

Average rotatable bonds  3.482758621

Number of hydrogen bond donors  40

Number of hydrogen bond acceptors  41

X logP energy  -5.79473

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.