General Information
SPKB ID SPKB07061
Signal sequence MARYTGPSWKLSRRLGISLSGTGKELEKRPYAP
Length 33
Organism Bacillus amyloliquefaciens 111018
UniProt ID NA,
Motif 1..33
Activity Information
Pathway Tat
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline protease yield: 10492.4(U/mL)
Type Tat/SPI
Structure Information
PDB ID 3J9W,
Predicted Structure ID SPKB07061.cif
Physicochemical Information
Molecular weight 3707.31164
Aliphatic index 65.15151515
Isoelectric point 11.11481821
Charge (pH=7) 4.99893537
Topological polar surface area 1546.04
Aromaticity 0.090909091
Average rotatable bonds 3.666666667
Number of hydrogen bond donors 57
Number of hydrogen bond acceptors 52
X logP energy -15.49942
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
