SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07056

Signal sequence   MAYDSRFDEWVQKLKEESFQNNTFDRRKFIQGAGKIAGLSLGLTIAQSVGAFEVNAA

Length  57

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..57



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline protease yield: 10963.9(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07056.cif

Structure  SPKB07056.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  6355.14864

Aliphatic index  75.43859649

Isoelectric point  6.736798472

Charge (pH=7)   0.004383759

Topological polar surface area  2702.83

Aromaticity  0.122807018

Average rotatable bonds  3.807017544

Number of hydrogen bond donors  93

Number of hydrogen bond acceptors  87

X logP energy  -27.61889

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.