SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07048

Signal sequence   MSRMDDKRFNCEKELTLAV

Length  19

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..19



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline protease yield: 12250(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  7BZD,

Predicted Structure ID  SPKB07048.cif

Structure  SPKB07048.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2286.66454

Aliphatic index  61.57894737

Isoelectric point  6.449600282

Charge (pH=7)   -0.060143893

Topological polar surface area  995.71

Aromaticity  0.052631579

Average rotatable bonds  4.263157895

Number of hydrogen bond donors  36

Number of hydrogen bond acceptors  34

X logP energy  -9.71646

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.