SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07040

Signal sequence   MSTRNSRSELEKTAVQLFRKLGTRTVLFHQAAAQAL

Length  36

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..36



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline protease yield: 13327.4(U/mL)

Type  Tat/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07040.cif

Structure  SPKB07040.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4060.69034

Aliphatic index  84.16666667

Isoelectric point  12.07913173

Charge (pH=7)   4.091840323

Topological polar surface area  1798.71

Aromaticity  0.055555556

Average rotatable bonds  3.888888889

Number of hydrogen bond donors  65

Number of hydrogen bond acceptors  58

X logP energy  -20.94332

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.