General Information
SPKB ID SPKB07035
Signal sequence MKKRKRRNFKRFIAAFLVLALMISLVPADVLA
Length 32
Organism Bacillus amyloliquefaciens 111018
UniProt ID NA,
Motif 1..32
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 14331.45(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07035.cif
Physicochemical Information
Molecular weight 3717.67154
Aliphatic index 128.125
Isoelectric point 12.53169954
Charge (pH=7) 6.997237403
Topological polar surface area 1408.93
Aromaticity 0.09375
Average rotatable bonds 4
Number of hydrogen bond donors 51
Number of hydrogen bond acceptors 46
X logP energy -6.53072
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
