SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07033

Signal sequence   MKKMLMLAFTFLLALTIHVGEASAV

Length  25

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..25



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline protease yield: 15674.89(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07033.cif

Structure  SPKB07033.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2736.42814

Aliphatic index  132.8

Isoelectric point  9.539374794

Charge (pH=7)   1.090078445

Topological polar surface area  940.43

Aromaticity  0.08

Average rotatable bonds  3.68

Number of hydrogen bond donors  33

Number of hydrogen bond acceptors  36

X logP energy  -3.0797

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.