SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07030

Signal sequence   MKKGIIRFLLVSFVLFFALSTGITGVQAAPA

Length  31

Organism  Bacillus amyloliquefaciens 111018

UniProt ID  NA,

Motif  1..31



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline protease yield: 18034.08(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07030.cif

Structure  SPKB07030.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3297.04844

Aliphatic index  129.0322581

Isoelectric point  11.82305527

Charge (pH=7)   2.99739092

Topological polar surface area  1165.48

Aromaticity  0.129032258

Average rotatable bonds  3.451612903

Number of hydrogen bond donors  41

Number of hydrogen bond acceptors  41

X logP energy  -6.03013

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.