SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07028

Signal sequence   MKNMSCKLVVSVTLFFSFLTIGPLAHAQ

Length  28

Organism  Bacillus subtilis WB600

UniProt ID  NA,

Motif  1..28



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline protease yield: 57.336(U/mL)

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07028.cif

Structure  SPKB07028.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3083.75864

Aliphatic index  107.8571429

Isoelectric point  9.677346754

Charge (pH=7)   2.026328607

Topological polar surface area  1108.28

Aromaticity  0.107142857

Average rotatable bonds  3.571428571

Number of hydrogen bond donors  39

Number of hydrogen bond acceptors  42

X logP energy  -7.8689

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.