SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB07027

Signal sequence   MRQGLMAAVLFATFALTGCGTDSAGK

Length  26

Organism  Bacillus subtilis WB600

UniProt ID  NA,

Motif  1..26



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  Alkaline protease yield: 64.98(U/mL)

Type  Sec/SPII



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB07027.cif

Structure  SPKB07027.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2618.07924

Aliphatic index  75.38461538

Isoelectric point  8.545113874

Charge (pH=7)   0.936157722

Topological polar surface area  1040.05

Aromaticity  0.076923077

Average rotatable bonds  3.307692308

Number of hydrogen bond donors  38

Number of hydrogen bond acceptors  38

X logP energy  -11.87373

Hydropathy 



Literature Information


Title  Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

DOI  10.1016/j.ijbiomac.2019.07.175,

Publication Year  2019

PubMed ID  31356949,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.