General Information
SPKB ID SPKB07026
Signal sequence MKKIVSILFMFGLVMGFSQFQPSTVFA
Length 27
Organism Bacillus subtilis WB600
UniProt ID O34310,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 112.12(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07026.cif
Physicochemical Information
Molecular weight 3054.76004
Aliphatic index 93.7037037
Isoelectric point 10.80537956
Charge (pH=7) 1.997394232
Topological polar surface area 1030.27
Aromaticity 0.185185185
Average rotatable bonds 3.703703704
Number of hydrogen bond donors 35
Number of hydrogen bond acceptors 39
X logP energy -4.4361
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
