General Information
SPKB ID SPKB07025
Signal sequence MKKWLIIAVSLAIAIVLFMYTKGEAKA
Length 27
Organism Bacillus subtilis WB600
UniProt ID O32258,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 146.53(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07025.cif
Physicochemical Information
Molecular weight 3009.78834
Aliphatic index 141.1111111
Isoelectric point 10.4849221
Charge (pH=7) 2.997728873
Topological polar surface area 1037.78
Aromaticity 0.111111111
Average rotatable bonds 3.777777778
Number of hydrogen bond donors 37
Number of hydrogen bond acceptors 38
X logP energy -1.4562
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
