General Information
SPKB ID SPKB07024
Signal sequence MRKKRVITCVMAASLTLGSLLPAGYASA
Length 28
Organism Bacillus subtilis WB600
UniProt ID P96657,
Motif 1..28
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 363(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07024.cif
Physicochemical Information
Molecular weight 2909.55994
Aliphatic index 108.2142857
Isoelectric point 10.88840969
Charge (pH=7) 3.934562479
Topological polar surface area 1137.45
Aromaticity 0.035714286
Average rotatable bonds 3.428571429
Number of hydrogen bond donors 43
Number of hydrogen bond acceptors 42
X logP energy -11.19376
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
