General Information
SPKB ID SPKB07023
Signal sequence MKRRKFSSVVAAVLIFALIFSLFSPGTKAAAA
Length 32
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..32
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Periplasmic space
Secretion efficiency Alkaline protease yield: 726.256(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07023.cif
Physicochemical Information
Molecular weight 3399.14444
Aliphatic index 110
Isoelectric point 12.54607567
Charge (pH=7) 4.997092575
Topological polar surface area 1259.64
Aromaticity 0.125
Average rotatable bonds 3.46875
Number of hydrogen bond donors 46
Number of hydrogen bond acceptors 44
X logP energy -9.11006
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
