General Information
SPKB ID SPKB07022
Signal sequence MNFKKTVVSALSISALALSVSGVASAH
Length 27
Organism Bacillus subtilis WB600
UniProt ID NA,
Motif 1..27
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 959(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07022.cif
Physicochemical Information
Molecular weight 2689.16574
Aliphatic index 119.2592593
Isoelectric point 10.80542203
Charge (pH=7) 2.088303323
Topological polar surface area 1085.34
Aromaticity 0.037037037
Average rotatable bonds 3.296296296
Number of hydrogen bond donors 39
Number of hydrogen bond acceptors 40
X logP energy -13.028
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
