General Information
SPKB ID SPKB07021
Signal sequence MKKRFSLIMMTGLLFGLTSPAFA
Length 23
Organism Bacillus subtilis WB600
UniProt ID P70960,
Motif 1..23
Activity Information
Pathway Sec
Natural/Artificial Artificial
Subcellular location Extracellular environment
Secretion efficiency Alkaline protease yield: 1050.36(U/mL)
Type Sec/SPI
Structure Information
PDB ID NA,
Predicted Structure ID SPKB07021.cif
Physicochemical Information
Molecular weight 2561.20024
Aliphatic index 93.47826087
Isoelectric point 11.82305527
Charge (pH=7) 2.99739092
Topological polar surface area 889.59
Aromaticity 0.130434783
Average rotatable bonds 3.695652174
Number of hydrogen bond donors 32
Number of hydrogen bond acceptors 34
X logP energy -4.64193
Hydropathy 
Literature Information
Title Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide
DOI 10.1016/j.ijbiomac.2019.07.175,
Publication Year 2019
PubMed ID 31356949,
Patent ID NA,
